| Gene | Trem2 |
| Chromosome | 17 |
| Deletion Start | 48,346,540 |
| Deletion End | 48,352,024 |
| Deletion Size | 5,485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,346,603 | 48,346,624 | CACAGGAGGGACCTACCTTCAG |
| TUP | 48,346,628 | 48,346,647 | CACTGTCTTTTCCTCACCTT |
| TUR | 48,346,649 | 48,346,668 | ACCCCAGCCATCTGCTCAAG |
| TDF | 48,351,682 | 48,351,702 | TCTCCACAGGCAGGTTTCATC |
| TDP | 48,351,706 | 48,351,733 | TGGGTCACCTCTAGCCTACCACCTTCCT |
| TDR | 48,351,750 | 48,351,770 | GGGTGGGAAGGAGGTCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GAAAGCAGCTGACCTTTACTG |
| SD | AGAAAGAGTAGGCAGTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 348 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 353 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10093A-A4 | VGB6 | C57BL/6NTac | |
| 10093B-B6 | VGB6 | C57BL/6NTac | |
| 10093B-C4 | VGB6 | C57BL/6NTac | |
| 10093B-H8 | VGB6 | C57BL/6NTac | |
