| Gene | Bmper |
| Chromosome | 9 |
| Deletion Start | 23,223,624 |
| Deletion End | 23,254,464 |
| Deletion Size | 30,841 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,223,807 | 23,223,825 | CGGGACTCTGGGTGGTTTC |
| TUP | 23,223,827 | 23,223,851 | CGGTCTGCCAGTGCCTCTTCTCCCA |
| TUR | 23,223,854 | 23,223,873 | GCGTTCCTCGTCCTCTCATC |
| TDF | 23,254,075 | 23,254,096 | TGAAAGGAAACCTTAGGATGGG |
| TDP | 23,254,099 | 23,254,124 | TGGAGCAAGTTCCTTCCTCAGCATGG |
| TDR | 23,254,136 | 23,254,156 | TGTCTTCAGATGCCAGCCTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGGGGCTGACGGCTAACC |
| SD | ATGTGCACAGGCGTCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 364 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10094A-C10 | VGB6 | C57BL/6NTac | |
| 10094B-A7 | VGB6 | C57BL/6NTac | |
| 10094B-A9 | VGB6 | C57BL/6NTac | |
| 10094C-D7 | VGB6 | C57BL/6NTac | |
| 10094C-E4 | VGB6 | C57BL/6NTac | |
| 10094C-E11 | VGB6 | C57BL/6NTac | |
