| Gene | Il24 |
| Chromosome | 1 |
| Deletion Start | 130,885,738 |
| Deletion End | 130,882,433 |
| Deletion Size | 3,306 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,885,336 | 130,885,357 | AAGAAGCCCCTGTGTGGTGTAG |
| TUP | 130,885,296 | 130,885,324 | TGGTCCTGGAACTCAGATCCAGAGCTCAT |
| TUR | 130,885,274 | 130,885,294 | TTGAAAGCCCTGCCTCTCATC |
| TDF | 130,882,805 | 130,882,825 | GCTCAGTGAGTTCCCCACATC |
| TDP | 130,882,773 | 130,882,801 | CCTAGCTGGCATCCAAGGTTCTTGTGTTC |
| TDR | 130,882,747 | 130,882,767 | AGGGCCAGAGAAGAACATGGT |
PCR Primers
| Primer | Sequence |
| SU | GTAGTCTCAAAGCAGAGTGC |
| SD | CTACTTTGTGGGTCAGTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 277 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10095A-E6 | VGB6 | C57BL/6NTac | |
| 10095A-E9 | VGB6 | C57BL/6NTac | |
| 10095B-A6 | VGB6 | C57BL/6NTac | |
| 10095B-F5 | VGB6 | C57BL/6NTac | |
| 10095B-D1 | VGB6 | C57BL/6NTac | |
