| Gene | Adamts15 |
| Chromosome | 9 |
| Deletion Start | 30,922,234 |
| Deletion End | 30,901,994 |
| Deletion Size | 20,241 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,921,878 | 30,921,897 | TTGCTGCTGTGAGCCTATGC |
| TUP | 30,921,838 | 30,921,861 | ACTCCGCACCTCGGTAGCCAAAGG |
| TUR | 30,921,816 | 30,921,835 | TTGGGCAGAGGGCTAATGAC |
| TDF | 30,902,285 | 30,902,305 | TGTGGCAGTGGCCTACAGAAG |
| TDP | 30,902,241 | 30,902,261 | CCCAGGGCAGCAAGGAGCCTC |
| TDR | 30,902,215 | 30,902,235 | ATGGCCGATGGTCTACATCAC |
PCR Primers
| Primer | Sequence |
| SU | ACCTCGCGTCACTTCTCCAC |
| SD | AGCCTGTGACCTCCATTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 399 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 431 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10097A-E4 | VGB6 | C57BL/6NTac | |
| 10097A-A3 | VGB6 | C57BL/6NTac | |
