| Gene | Gpr119 |
| Chromosome | X |
| Deletion Start | 48,674,255 |
| Deletion End | 48,673,230 |
| Deletion Size | 1,026 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,673,820 | 48,673,841 | CCACTCGGAGTCTCCATATTCC |
| TUP | 48,673,793 | 48,673,817 | CAGACCACCTACCATGGACCCTGCA |
| TUR | 48,673,770 | 48,673,791 | TGGGTGAAACACAGCAAAGAAG |
| TDF | 48,673,335 | 48,673,358 | TTCACTTCAATCCTCCTCCTTCTC |
| TDP | 48,673,302 | 48,673,328 | TCTGGTCCTCTCTGGACCACGATTCCT |
| TDR | 48,673,271 | 48,673,294 | GGCTGATAGTGACGATGTGATAGG |
PCR Primers
| Primer | Sequence |
| SU | AGAAAAGCCGGAGGCTGAGG |
| SD | ACAGCATATATCTGGTATAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 402 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10098A-A4 | VGB6 | C57BL/6NTac | |
| 10098B-A9 | VGB6 | C57BL/6NTac | |
| 10098B-B8 | VGB6 | C57BL/6NTac | |
