| Gene | Il20ra |
| Chromosome | 10 |
| Deletion Start | 19,742,903 |
| Deletion End | 19,759,653 |
| Deletion Size | 16,751 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,743,547 | 19,743,567 | AAACCGTGCAGAATTCAATGC |
| TUP | 19,743,570 | 19,743,595 | TGGCTCGCTCCCACTAAACAACCACA |
| TUR | 19,743,616 | 19,743,635 | TGCCTGTGAAGGGTCATGAC |
| TDF | 19,759,102 | 19,759,122 | GGATGCTCTTCGCATTTGATG |
| TDP | 19,759,125 | 19,759,149 | TCTTTGCTCAGCACCACAGACAGCG |
| TDR | 19,759,164 | 19,759,182 | CCACCCATGCTGGGTTAGG |
PCR Primers
| Primer | Sequence |
| SU | AACTTCAAAGCTACCTATGCC |
| SD | AAAGTCCCCACCCAACCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 436 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10099A-C2 | VGB6 | C57BL/6NTac | |
| 10099A-A5 | VGB6 | C57BL/6NTac | |
