| Gene | Gpr150 |
| Chromosome | 13 |
| Deletion Start | 76,056,821 |
| Deletion End | 76,055,521 |
| Deletion Size | 1,301 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,056,797 | 76,056,817 | ATCCTTTCAGCCTCGCAATTC |
| TUP | 76,056,767 | 76,056,791 | CCCGCATCCAACCTTTCTGTACCCA |
| TUR | 76,056,719 | 76,056,739 | CAGAGGCTCCTTGCTCAGAAG |
| TDF | 76,056,549 | 76,056,568 | AGCGTCGCAAGATGGACTTC |
| TDP | 76,056,510 | 76,056,534 | ACTTGCGTACAGGTCAGCCGCTGCT |
| TDR | 76,056,486 | 76,056,505 | CAGCTGCGACAATGCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | ATACCAAGAACAACCTATAC |
| SD | TGGTCTCCTCAAAATGGGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 295 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 308 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10101C-A7 | VGB6 | C57BL/6NTac | |
| 10101C-B7 | VGB6 | C57BL/6NTac | |
| 10101C-B10 | VGB6 | C57BL/6NTac | |
| 10101C-B9 | VGB6 | C57BL/6NTac | |
