| Gene | Tmem20 |
| Chromosome | 19 |
| Deletion Start | 38,396,098 |
| Deletion End | 38,403,378 |
| Deletion Size | 7,281 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,396,747 | 38,396,767 | AAAGAGAGGCCGTGGAAGATG |
| TUP | 38,396,774 | 38,396,796 | CGGGATTCGGTCTCACCCCACCT |
| TUR | 38,402,761 | 38,402,782 | CGGGACAGCTAAACGCAATAAC |
| TDF | 38,402,702 | 38,402,721 | CCAGTGCCATGATCCTCATG |
| TDP | 38,402,734 | 38,402,756 | CAGACAACGTCCCTCGCAGACGC |
| TDR | 38,402,761 | 38,402,782 | CGGGACAGCTAAACGCAATAAC |
PCR Primers
| Primer | Sequence |
| SU | CCAGGGCTGAGAACCAAGCG |
| SD | TACTCTTAACTAAAAACGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 433 bp | - | + | + |
| TUF | TUR | 6036 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 248 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10103D-A8 | VGB6 | C57BL/6NTac | |
| 10103D-A9 | VGB6 | C57BL/6NTac | |
| 10103D-E9 | VGB6 | C57BL/6NTac | |
| 10103D-D4 | VGB6 | C57BL/6NTac | |
| 10103D-F2 | VGB6 | C57BL/6NTac | |
