| Gene | Gpr158 |
| Chromosome | 2 |
| Deletion Start | 21,368,239 |
| Deletion End | 21,399,367 |
| Deletion Size | 31,129 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,368,649 | 21,368,669 | GCCACCAACTTCCTCAACATG |
| TUP | 21,368,681 | 21,368,707 | CAATAAGTCTCGGGAACAGACCGTGCA |
| TUR | 21,368,740 | 21,368,760 | GCTCACCCTCCAGGAGACTTC |
| TDF | 21,398,694 | 21,398,713 | GCTTTGTTGGCAGCAGTTTG |
| TDP | 21,398,715 | 21,398,744 | CAACCGCTCTTGAATGTACCGTGTTAGCTG |
| TDR | 21,398,746 | 21,398,766 | AAGAACCTGCACTTGCTTTGG |
PCR Primers
| Primer | Sequence |
| SU | TGCGAGGTGCGTAATCCCGAG |
| SD | CATTTTGGAGAATAAATTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 422 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10108A-A5 | VGB6 | C57BL/6NTac | |
| 10108C-F3 | VGB6 | C57BL/6NTac | |
