10110   Lrrc4c     Status: ES cell colonies screened / QC no positives

GeneLrrc4c
Chromosome2
Deletion Start97,629,015
Deletion End97,630,950
Deletion Size1,936
Genome Build38
MGI IDMGI:2442636
NCBI Gene ID241568
BAC ID379E3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,629,13797,629,155TTGTGGTGGCTGGTTTGGT
TUP97,629,15997,629,183TGCTCAAACCTGCCCTTCAGTGTGC
TUR97,629,19097,629,210CACCTTGCTGAACTGGTTGCT
TDF97,630,62197,630,640GGGTGTTTTGTGGCCATCAC
TDP97,630,64497,630,668AATGACCAGCATCACGGCAGCCATG
TDR97,630,68397,630,702TTTGCCGATGGTGCTGTTTC

PCR Primers

PrimerSequence
SUTTGGACTACTGACATCTTTC
SDAAATAGACTGGATCTCACCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev365 bp-++
TUFTUR74 bp++-
TDFTDR82 bp++-
NeoFwdSD452 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10110C-A10VGB6C57BL/6NTac
10110C-E12VGB6C57BL/6NTac
10110C-E6VGB6C57BL/6NTac
10110C-E7VGB6C57BL/6NTac
10110C-F10VGB6C57BL/6NTac
10110C-C12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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