| Gene | D630039A03Rik |
| Chromosome | 4 |
| Deletion Start | 57,915,792 |
| Deletion End | 57,910,078 |
| Deletion Size | 5,715 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,915,638 | 57,915,660 | GCCCAGTATGAAGGTCTGAGGAA |
| TUP | 57,915,611 | 57,915,636 | CAGCAGAGGACCCAGGCCCACCTAAT |
| TUR | 57,915,582 | 57,915,602 | CACGCCCAGCCCTACCTTTAG |
| TDF | 57,910,680 | 57,910,701 | AGGGTCTGGCCTTCCTTTAATC |
| TDP | 57,910,644 | 57,910,670 | TGAGTTTTCCTCAAAGGCAGACCTCCC |
| TDR | 57,910,620 | 57,910,640 | AGAAAGGACAGCAGCGTCATC |
PCR Primers
| Primer | Sequence |
| SU | AATAAAAGCTCCCTGGACTG |
| SD | ATAAGATTAAAGTTGGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 519 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10111A-A4 | VGB6 | C57BL/6NTac | |
| 10111A-B12 | VGB6 | C57BL/6NTac | |
| 10111A-C1 | VGB6 | C57BL/6NTac | |
| 10111A-D12 | VGB6 | C57BL/6NTac | |
| 10111A-F10 | VGB6 | C57BL/6NTac | |
| 10111A-F12 | VGB6 | C57BL/6NTac | |
