| Gene | Elfn1 |
| Chromosome | 5 |
| Deletion Start | 139,971,226 |
| Deletion End | 139,973,729 |
| Deletion Size | 2,504 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,971,321 | 139,971,341 | GGTGACTGCTGGCTGATTGAG |
| TUP | 139,971,353 | 139,971,376 | CTGCAGATGGCCAACCACACGAAG |
| TUR | 139,971,378 | 139,971,398 | CTCATAGGGTGGCTGGTTCTG |
| TDF | 139,973,601 | 139,973,621 | CACAAGGATGATGCGGAGTTC |
| TDP | 139,973,636 | 139,973,659 | CGCCCTGCGCAAGAAGGTTCAGTT |
| TDR | 139,973,663 | 139,973,683 | TCATGCAGGTCCTCGTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCAGCAGAGAGTACTTCAG |
| SD | ACCAGGCAGCAGGCACATTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10112A-C9 | VGB6 | C57BL/6NTac | |
| 10112C-B9 | VGB6 | C57BL/6NTac | |
| 10112C-F5 | VGB6 | C57BL/6NTac | |
| 10112D-B12 | VGB6 | C57BL/6NTac | |
