| Gene | Clec1a |
| Chromosome | 6 |
| Deletion Start | 129,451,712 |
| Deletion End | 129,428,837 |
| Deletion Size | 22,876 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,451,574 | 129,451,592 | TGTCTCCCCGAATCCAAGC |
| TUP | 129,451,538 | 129,451,566 | TGCCTCATCTCACGATTTTCTCCAGAGCT |
| TUR | 129,451,513 | 129,451,532 | CAGCCTTGCTCATGCCTAGG |
| TDF | 129,428,946 | 129,428,969 | GGGACTGTATGACCATCTTCAATG |
| TDP | 129,428,926 | 129,428,944 | AAAGGCTTACTCTAAGGAC |
| TDR | 129,428,904 | 129,428,924 | CACACCTCCTCAGCTCTTTGC |
PCR Primers
| Primer | Sequence |
| SU | TCACATCCCCACCTTGCAAC |
| SD | TGGGGCTAGTCGGAGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 450 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | 322 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10113H-D4 | VGB6 | C57BL/6NTac | |
| 10113H-H6 | VGB6 | C57BL/6NTac | |
