| Gene | Lrfn4 |
| Chromosome | 19 |
| Deletion Start | 4,614,501 |
| Deletion End | 4,612,057 |
| Deletion Size | 2,445 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,614,346 | 4,614,365 | ACAGGCGCACAGTTGAACTG |
| TUP | 4,614,315 | 4,614,338 | CCGACAACTTCATCCAGGCCCTGG |
| TUR | 4,614,286 | 4,614,306 | CCCTGTCATGTTGCGGAAGTC |
| TDF | 4,612,517 | 4,612,536 | CGACCTCACGGCCACTAGAC |
| TDP | 4,612,488 | 4,612,513 | TGGGCTGTGCCCACTTCTCTACGCTA |
| TDR | 4,612,445 | 4,612,463 | CCAGCACATGGGCCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GGGAAATGTGACCTTTACTC |
| SD | AACAAGACTCCCTCTTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 324 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 341 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10115A-A2 | VGB6 | C57BL/6NTac | |
| 10115A-A9 | VGB6 | C57BL/6NTac | |
| 10115A-A11 | VGB6 | C57BL/6NTac | |
| 10115A-B2 | VGB6 | C57BL/6NTac | |
| 10115A-B8 | VGB6 | C57BL/6NTac | |
| 10115A-D7 | VGB6 | C57BL/6NTac | |
