| Gene | Neto1 |
| Chromosome | 18 |
| Deletion Start | 86,395,866 |
| Deletion End | 86,404,828 |
| Deletion Size | 8,963 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,395,920 | 86,395,941 | TCTCCAGAGTGCCAGTCAGTTC |
| TUP | 86,395,948 | 86,395,972 | TGCTGCCACTTCTCCTCTTGGACCC |
| TUR | 86,395,981 | 86,396,001 | AGTGGTTGGTAGGGCTTTTCC |
| TDF | 86,404,387 | 86,404,408 | AGCATTGCTGACTTGGTGTTTG |
| TDP | 86,404,412 | 86,404,429 | CCTGTCAGGTATTTCACA |
| TDR | 86,404,433 | 86,404,453 | GGCCTGAAACCGATGATGTTG |
PCR Primers
| Primer | Sequence |
| SU | GGTGCTCATGGTGGCGGTCG |
| SD | GTAAAGCATATGGAAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 394 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10116A-E4 | VGB6 | C57BL/6NTac | |
| 10116A-E10 | VGB6 | C57BL/6NTac | |
| 10116B-G10 | VGB6 | C57BL/6NTac | |
