| Gene | Creg2 |
| Chromosome | 1 |
| Deletion Start | 39,650,937 |
| Deletion End | 39,623,077 |
| Deletion Size | 27,861 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,650,424 | 39,650,444 | CCGGTTTCACAGGTTCATAGG |
| TUP | 39,650,392 | 39,650,417 | ATGCCCTGTGAATAACGGAACCAGCG |
| TUR | 39,650,348 | 39,650,367 | TCCCCACTCACATGCACTTG |
| TDF | 39,623,362 | 39,623,383 | CCACCCTCAGCTCATCAGATTC |
| TDP | 39,623,345 | 39,623,360 | ACCTCTGCCTGGGTTG |
| TDR | 39,623,321 | 39,623,342 | GCCTGATTTGGGTTGAGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTCTGGCTGCAAAACGCTTG |
| SD | ACGGCGTACGGCATAAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10118A-H2 | VGB6 | C57BL/6NTac | |
| 10118D-G12 | VGB6 | C57BL/6NTac | |
| 10118E-A3 | VGB6 | C57BL/6NTac | |
