| Gene | Acsl5 |
| Chromosome | 19 |
| Deletion Start | 55,268,656 |
| Deletion End | 55,295,513 |
| Deletion Size | 26,858 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,269,661 | 55,269,680 | AAGGTTTTCTGGGCCACATG |
| TUP | 55,269,682 | 55,269,707 | ACCTGGAGCTGGCCTCCACTTAATGC |
| TUR | 55,269,717 | 55,269,736 | CAGGTCGCTGGTGCTTTCAC |
| TDF | 55,295,388 | 55,295,407 | GTGCATCCAGAGCCCTTCAC |
| TDP | 55,295,411 | 55,295,439 | TGAAAACGGACTTCTGACACCGACACTGA |
| TDR | 55,295,443 | 55,295,463 | TGGCAAGCTCTACTCGTTTGG |
PCR Primers
| Primer | Sequence |
| SU | TGGCCTTCTGGGTTCTGTGG |
| SD | CAGTCGTGAGGAAGTGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 499 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10120B-B3 | VGB6 | C57BL/6NTac | |
| 10120C-D7 | VGB6 | C57BL/6NTac | |
| 10120E-E8 | VGB6 | C57BL/6NTac | |
| 10120E-C1 | VGB6 | C57BL/6NTac | |
| 10120F-C6 | VGB6 | C57BL/6NTac | |
| 10120G-G3 | VGB6 | C57BL/6NTac | |
| 10120G-A10 | VGB6 | C57BL/6NTac | |
| 10120F-E10 | VGB6 | C57BL/6NTac | |
