| Gene | Tmem26 |
| Chromosome | 10 |
| Deletion Start | 68,723,884 |
| Deletion End | 68,751,268 |
| Deletion Size | 27,385 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,723,939 | 68,723,959 | CTGCTGTTCCTGTTGCATTCC |
| TUP | 68,723,964 | 68,723,990 | TCACCCAGGTCACTCTCCAGACGGCGA |
| TUR | 68,723,993 | 68,724,013 | CAGCCAGTAACGGTGCTCTTC |
| TDF | 68,751,099 | 68,751,120 | GTTCAACAGGCCAGAGGCTTTG |
| TDP | 68,751,122 | 68,751,152 | CGACAATTTATCCATGGTATGTGAACCGGTC |
| TDR | 68,751,157 | 68,751,180 | GGAGTGTTTGGTGGAGTCCTAAGG |
PCR Primers
| Primer | Sequence |
| SU | GTCCCAGTTGCCTGCAGAGC |
| SD | AAGCCCTGACATAAAGCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 375 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 275 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10123B-C11 | VGB6 | C57BL/6NTac | |
| 10123B-F6 | VGB6 | C57BL/6NTac | |
