10124 Gpr111 Status: ES cell colonies screened / QC no positives
| Gene | Gpr111 |
| Chromosome | 17 |
| Deletion Start | 42,713,152 |
| Deletion End | 42,709,972 |
| Deletion Size | 3,181 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,713,066 | 42,713,087 | CAATGAGGAAAAGATGCAGGTG |
| TUP | 42,713,040 | 42,713,063 | CCTACTGTCCGGTCTGCAAGGCCA |
| TUR | 42,713,013 | 42,713,035 | ACCTAAGTTCCTTCCCCAATGAG |
| TDF | 42,710,410 | 42,710,428 | GGCTATGGGTGCCCTTTGG |
| TDP | 42,710,381 | 42,710,408 | CATCGCCATTATCACACTTGCCGTTACA |
| TDR | 42,710,356 | 42,710,374 | GCCGGAGGTAGCCTTTTCC |
PCR Primers
| Primer | Sequence |
| SU | AACGGAAGCATATTGTGTGC |
| SD | ATCCTCAGCCTATTAACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 389 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
