| Gene | Ldlrad2 |
| Chromosome | 4 |
| Deletion Start | 137,574,566 |
| Deletion End | 137,571,582 |
| Deletion Size | 2,985 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,574,380 | 137,574,398 | AGGAGGGAGCGTGGCTACA |
| TUP | 137,574,357 | 137,574,376 | ATGGTCATTTTAGAGATCAA |
| TUR | 137,574,337 | 137,574,355 | CGCTGCACTGACCCTCACT |
| TDF | 137,572,259 | 137,572,278 | AATGCTGCTGACCTGCACTG |
| TDP | 137,572,233 | 137,572,255 | TCCTCTGGTGCTGCTGTTGCCCA |
| TDR | 137,572,187 | 137,572,205 | ATCTGAGGCGGAGCCTGTG |
PCR Primers
| Primer | Sequence |
| SU | ACCACAACCAGTTTTCACTG |
| SD | TAACGTCCCAGTTAGTGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 341 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10127A-A1 | VGB6 | C57BL/6NTac | |
| 10127A-C2 | VGB6 | C57BL/6NTac | |
| 10127A-D8 | VGB6 | C57BL/6NTac | |
| 10127A-E8 | VGB6 | C57BL/6NTac | |
| 10127A-H1 | VGB6 | C57BL/6NTac | |
