| Gene | Angptl7 |
| Chromosome | 4 |
| Deletion Start | 148,500,286 |
| Deletion End | 148,496,155 |
| Deletion Size | 4,152 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,500,265 | 148,500,285 | TGAGGGAGACCTGGCTATGTG |
| TUP | 148,500,235 | 148,500,260 | CCTTGTAGCCTTTGTCAGCCACCCAG |
| TUR | 148,500,205 | 148,500,226 | TCTTGCGTTTATGAGGCTTCTG |
| TDF | 148,496,234 | 148,496,254 | AAAGCACATGGATGGCATCAG |
| TDP | 148,496,208 | 148,496,232 | AGTTGGCTCCATGCCAGCCATACCA |
| TDR | 148,496,178 | 148,496,200 | GGATCTTCATTTCCACACGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCACTCAACAGGCACCTGAC |
| SD | TAGGGAAAATGGGTTAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 407 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 353 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10128A-G6 | VGB6 | C57BL/6NTac | |
