| Gene | Egfl8 |
| Chromosome | 17 |
| Deletion Start | 34,615,264 |
| Deletion End | 34,613,538 |
| Deletion Size | 1,727 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,614,822 | 34,614,840 | GGACCCCAAGAGGCAAGTC |
| TUP | 34,614,792 | 34,614,810 | CCTACAGATATGAGCCTGC |
| TUR | 34,614,757 | 34,614,775 | CCAGGCCACACGGTATGTG |
| TDF | 34,613,844 | 34,613,863 | AACAGGTGGCTGAGCTTTGG |
| TDP | 34,613,807 | 34,613,832 | CAGAATTGAGTCCCTCAGCGACCAGG |
| TDR | 34,613,732 | 34,613,752 | GAGGCTGGGTCTTCAGTCATG |
PCR Primers
| Primer | Sequence |
| SU | CAGGAGCATTTAGAGTGGTG |
| SD | AACCCAGGAGGTAGACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10130A-G12 | VGB6 | C57BL/6NTac | |
| 10130A-E8 | VGB6 | C57BL/6NTac | |
| 10130B-B2 | VGB6 | C57BL/6NTac | |
| 10130B-C8 | VGB6 | C57BL/6NTac | |
| 10130B-E8 | VGB6 | C57BL/6NTac | |
| 10130B-H6 | VGB6 | C57BL/6NTac | |
