| Gene | Gpnmb |
| Chromosome | 6 |
| Deletion Start | 49,036,708 |
| Deletion End | 49,056,314 |
| Deletion Size | 19,607 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,036,847 | 49,036,866 | GCCCTTTGACTGGAGTTTGG |
| TUP | 49,036,878 | 49,036,904 | CACTCCCAGCATTTGGAACAGACCCTG |
| TUR | 49,036,913 | 49,036,933 | TGCTCTCCGTGAGTCCTAAGC |
| TDF | 49,056,194 | 49,056,212 | CCCCAGGAACACGGTCAAG |
| TDP | 49,056,221 | 49,056,246 | CCTGAGTGTTCTCCTCAGTCACGCGA |
| TDR | 49,056,254 | 49,056,274 | TCCTGGTCTCCTCGGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCAAGTGACTCATGAGCACG |
| SD | GAGGCATCTGTGGTATCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 319 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 378 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10131A-A10 | VGB6 | C57BL/6NTac | |
| 10131A-F10 | VGB6 | C57BL/6NTac | |
| 10131A-H2 | VGB6 | C57BL/6NTac | |
| 10131A-H10 | VGB6 | C57BL/6NTac | |
| 10131A-H11 | VGB6 | C57BL/6NTac | |
| 10131A-D7 | VGB6 | C57BL/6NTac | |
