| Gene | Ano4 |
| Chromosome | 10 |
| Deletion Start | 89,084,632 |
| Deletion End | 89,068,150 |
| Deletion Size | 16,483 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,084,550 | 89,084,570 | CCGCCGGTACAAGTTCATGAG |
| TUP | 89,084,509 | 89,084,538 | CAGCAGACAACACCATCAGTACAGAGCATG |
| TUR | 89,084,482 | 89,084,504 | TCCTTGCCATTGTTAGGGTACAG |
| TDF | 89,068,303 | 89,068,322 | GTGGAGGGCATGGAGTCATC |
| TDP | 89,068,269 | 89,068,299 | AGTCAACATCACTCTGAAACACAGGAGCCAT |
| TDR | 89,068,240 | 89,068,260 | CATGGAAGGCAAGATCAAAGG |
PCR Primers
| Primer | Sequence |
| SU | ATGCATGCAAAATACTACTC |
| SD | CAAAACAAAAAACATTGGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 338 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10134A-E1 | VGB6 | C57BL/6NTac | |
| 10134A-F3 | VGB6 | C57BL/6NTac | |
| 10134A-H7 | VGB6 | C57BL/6NTac | |
| 10134B-D8 | VGB6 | C57BL/6NTac | |
| 10134B-D9 | VGB6 | C57BL/6NTac | |
