| Gene | Tmem174 |
| Chromosome | 13 |
| Deletion Start | 98,637,317 |
| Deletion End | 98,636,387 |
| Deletion Size | 931 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,637,008 | 98,637,029 | TGCCAGTTGTGCTCAGATAACG |
| TUP | 98,636,966 | 98,636,990 | ACTGTCCTCCGGAAGTCTGGTCCGA |
| TUR | 98,636,941 | 98,636,961 | TGATTGATGCCAGTGAAAACG |
| TDF | 98,636,882 | 98,636,902 | TCCCTCCTCCTTACGGTTCTC |
| TDP | 98,636,851 | 98,636,875 | CCCTGGGAATGAACGCCACCTACCT |
| TDR | 98,636,824 | 98,636,844 | AGACCGCAAGGATTCATCATG |
PCR Primers
| Primer | Sequence |
| SU | GCAGATTGGTACTGTTCCTC |
| SD | TCACTCTATCACAGAGCGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 330 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10136A-B8 | VGB6 | C57BL/6NTac | |
| 10136A-G1 | VGB6 | C57BL/6NTac | |
| 10136A-F11 | VGB6 | C57BL/6NTac | |
| 10136A-F4 | VGB6 | C57BL/6NTac | |
| 10136A-E5 | VGB6 | C57BL/6NTac | |
| 10136A-B7 | VGB6 | C57BL/6NTac | |
