| Gene | Igfbp5 |
| Chromosome | 1 |
| Deletion Start | 72,874,133 |
| Deletion End | 72,862,004 |
| Deletion Size | 12,130 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,874,085 | 72,874,103 | TATGCCGTACCGGCTCAAG |
| TUP | 72,874,056 | 72,874,082 | CTGGGTTCTTTCGTGCACTGTGAACCC |
| TUR | 72,874,033 | 72,874,053 | ATGGACAGAGCTTTCTCGTCG |
| TDF | 72,862,521 | 72,862,540 | GGGTATCTCACAGCCGCTTG |
| TDP | 72,862,493 | 72,862,518 | TCTCTCCCAGTGTAAGCCCTCCCGTG |
| TDR | 72,862,471 | 72,862,489 | CAGCAGATGCCACGTTTGC |
PCR Primers
| Primer | Sequence |
| SU | CTTTTCAAGGCCTCCAAGCT |
| SD | CTGAGTTCACTTGTCTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 485 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 399 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10138A-C1 | VGB6 | C57BL/6NTac | |
| 10138B-E12 | VGB6 | C57BL/6NTac | |
