| Gene | Esm1 |
| Chromosome | 13 |
| Deletion Start | 113,209,833 |
| Deletion End | 113,216,750 |
| Deletion Size | 6,918 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,209,872 | 113,209,891 | CCTGGTACCTCTGCACCTGG |
| TUP | 113,209,893 | 113,209,915 | CATATTTGGCGCTCCAGGCCATG |
| TUR | 113,209,917 | 113,209,934 | TGTTCCGGGCAATCCACC |
| TDF | 113,216,627 | 113,216,647 | CTTCCGATTTCAGAGCGTGAC |
| TDP | 113,216,653 | 113,216,676 | CTCACAGCGTTGCCATCTCCGGAT |
| TDR | 113,216,681 | 113,216,701 | AGCGTTCCCTTCTCCAATCTC |
PCR Primers
| Primer | Sequence |
| SU | TTCAATTCCTGGATGGCAGC |
| SD | ATTGGATTACTCATCGACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10141C-A3 | VGB6 | C57BL/6NTac | |
| 10141C-F9 | VGB6 | C57BL/6NTac | |
| 10141C-G5 | VGB6 | C57BL/6NTac | |
| 10141D-D2 | VGB6 | C57BL/6NTac | |
