| Gene | Cd163 |
| Chromosome | 6 |
| Deletion Start | 124,304,738 |
| Deletion End | 124,329,373 |
| Deletion Size | 24,636 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,305,529 | 124,305,549 | GCTCAGGCTTAAGAAGCTGCC |
| TUP | 124,305,558 | 124,305,582 | TTGCCTCCATGCCCAGCGTTTTCAC |
| TUR | 124,305,590 | 124,305,608 | TACGTGCAGCCCAGGAGTG |
| TDF | 124,328,713 | 124,328,732 | TCATTCCAGGAGAAGTGCCC |
| TDP | 124,328,738 | 124,328,764 | TTCATTGTCCTCACTCCTCGGGAACCT |
| TDR | 124,328,766 | 124,328,785 | CCAAGGCAAATCCCTCTCAG |
PCR Primers
| Primer | Sequence |
| SU | GTGTTCCAAAGTGGGAGGAG |
| SD | ATGAAGATGCAGCTGTGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 332 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 379 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10143B-A3 | VGB6 | C57BL/6NTac | |
| 10143B-F4 | VGB6 | C57BL/6NTac | |
| 10143C-F11 | VGB6 | C57BL/6NTac | |
| 10143E-B5 | VGB6 | C57BL/6NTac | |
| 10143F-E2 | VGB6 | C57BL/6NTac | |
| 10143F-B6 | VGB6 | C57BL/6NTac | |
