| Gene | Il17re |
| Chromosome | 6 |
| Deletion Start | 113,458,879 |
| Deletion End | 113,470,143 |
| Deletion Size | 11,265 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,459,222 | 113,459,241 | CAGCTGGATGGATGGACTGA |
| TUP | 113,459,244 | 113,459,269 | CCAACCATCTGGGTCTGAGACCCTCA |
| TUR | 113,459,274 | 113,459,294 | GGTGCATTCTCCCTGCACTCT |
| TDF | 113,469,223 | 113,469,242 | CCCCTAGTGGGAAGGAATGG |
| TDP | 113,469,245 | 113,469,268 | ACCCACAGCCCTTTGCCCTGCCTT |
| TDR | 113,469,280 | 113,469,300 | GCAGGGTGGATGGTGGTACAC |
PCR Primers
| Primer | Sequence |
| SU | CAAGCAAGTGTTCCTCAGTG |
| SD | GTCCCTCCTTCTCAGAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 287 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10148B-C3 | VGB6 | C57BL/6NTac | |
| 10148B-D8 | VGB6 | C57BL/6NTac | |
| 10148B-E7 | VGB6 | C57BL/6NTac | |
| 10148B-G1 | VGB6 | C57BL/6NTac | |
| 10148B-G9 | VGB6 | C57BL/6NTac | |
| 10148B-H10 | VGB6 | C57BL/6NTac | |
