| Gene | Resp18 |
| Chromosome | 1 |
| Deletion Start | 75,278,304 |
| Deletion End | 75,272,291 |
| Deletion Size | 6,014 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,278,260 | 75,278,279 | CCGGGCATCTTCAGCTACTG |
| TUP | 75,278,225 | 75,278,252 | TCCTTCTGCTTTACAGTCGTCCAGGCAG |
| TUR | 75,278,198 | 75,278,217 | TGACCGTGGGCGTTTATGTC |
| TDF | 75,272,731 | 75,272,752 | GGTCCCAGCCCTTGATACTTTC |
| TDP | 75,272,697 | 75,272,722 | CAAGCTCCCATTTCTGCAGCATGACA |
| TDR | 75,272,663 | 75,272,686 | CCCCAGCAACTAATTCAGGTTTAG |
PCR Primers
| Primer | Sequence |
| SU | GGAAAAGTGTGTAGAGGGTG |
| SD | TGGTCACCTCTCACACTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 361 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10152A-A5 | VGB6 | C57BL/6NTac | |
| 10152A-C10 | VGB6 | C57BL/6NTac | |
| 10152A-D9 | VGB6 | C57BL/6NTac | |
| 10152A-F7 | VGB6 | C57BL/6NTac | |
| 10152A-G1 | VGB6 | C57BL/6NTac | |
| 10152A-G2 | VGB6 | C57BL/6NTac | |
