| Gene | Scg2 |
| Chromosome | 1 |
| Deletion Start | 79,437,001 |
| Deletion End | 79,435,130 |
| Deletion Size | 1,872 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,436,617 | 79,436,636 | TGAAAATGAGCCGCCATCTG |
| TUP | 79,436,589 | 79,436,615 | CCCCAAAGAGAACAAGCCCTATGCCTT |
| TUR | 79,436,557 | 79,436,576 | GCGTGTCCACTGGGAAGTTC |
| TDF | 79,435,752 | 79,435,772 | GCTCTCCAAGGGTGGGTATCC |
| TDP | 79,435,718 | 79,435,746 | CAAGGCCTCTACCATACCACGACCAGGTG |
| TDR | 79,435,696 | 79,435,716 | CCTCGACACTCAGCCCATCAG |
PCR Primers
| Primer | Sequence |
| SU | GACAAGCTTGATGCAAATGAG |
| SD | ACATTAGCCAACACTATCATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 342 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10153C-H1 | VGB6 | C57BL/6NTac | |
| 10153A-B6 | VGB6 | C57BL/6NTac | |
| 10153B-D10 | VGB6 | C57BL/6NTac | |
| 10153B-F4 | VGB6 | C57BL/6NTac | |
