| Gene | Prpf18 |
| Chromosome | 2 |
| Deletion Start | 4,651,978 |
| Deletion End | 4,624,446 |
| Deletion Size | 27,553 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,651,886 | 4,651,904 | AGCTGTGGTTCGGGCTTGA |
| TUP | 4,651,859 | 4,651,882 | CAACACCGCAAGCACAGTCCACCA |
| TUR | 4,651,829 | 4,651,849 | CAGACCCTCTCAGGACCATCA |
| TDF | 4,625,098 | 4,625,121 | TTGTCACCTTGAATGGTTTTCTTG |
| TDP | 4,625,068 | 4,625,096 | TCATTTAGGGCTCACTTCTCTCTGGCAGC |
| TDR | 4,625,037 | 4,625,064 | GCATAGATAATAACTGGCTCTCCTCAAC |
PCR Primers
| Primer | Sequence |
| SU | TTCTACCTGTTACTGAAATC |
| SD | CAACCCCCTTTCCTGAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 392 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 381 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10155A-B10 | VGB6 | C57BL/6NTac | |
| 10155A-F8 | VGB6 | C57BL/6NTac | |
| 10155A-G2 | VGB6 | C57BL/6NTac | |
| 10155A-G11 | VGB6 | C57BL/6NTac | |
