| Gene | Tmem81 |
| Chromosome | 1 |
| Deletion Start | 132,507,440 |
| Deletion End | 132,508,274 |
| Deletion Size | 835 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,507,666 | 132,507,685 | ACTGCACGTTCCAGCGTTTG |
| TUP | 132,507,689 | 132,507,715 | CATCCCACAGATCCAGTTGGTCACACA |
| TUR | 132,507,718 | 132,507,738 | CCATGGACGATGGTGAAATGG |
| TDF | 132,508,120 | 132,508,139 | GACATCGGCCTTGGGCATAG |
| TDP | 132,508,141 | 132,508,164 | CCGCCAACCACTCCAGCCACAATT |
| TDR | 132,508,166 | 132,508,186 | GACAGCAACGCTCACCAACAC |
PCR Primers
| Primer | Sequence |
| SU | AGCTTAGAGAGTCAAGCGAC |
| SD | ACAGAACTCTCTCTAGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 332 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 278 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10156C-G11 | VGB6 | C57BL/6NTac | |
| 10156C-B10 | VGB6 | C57BL/6NTac | |
| 10156D-H3 | VGB6 | C57BL/6NTac | |
| 10156D-B4 | VGB6 | C57BL/6NTac | |
