| Gene | Entpd8 |
| Chromosome | 2 |
| Deletion Start | 25,081,806 |
| Deletion End | 25,085,152 |
| Deletion Size | 3,347 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,081,850 | 25,081,870 | TCATGGCTCTGTTGGGAGTTG |
| TUP | 25,081,875 | 25,081,898 | AGCCTCTGGCCTCACCATGCTCGT |
| TUR | 25,081,900 | 25,081,920 | GATTGCCTTCACCAGGATGAG |
| TDF | 25,084,985 | 25,085,005 | ACACTGGGCTTCATGCTGAAC |
| TDP | 25,085,018 | 25,085,041 | ATTCCAGCTGAGGCACCGACCCAC |
| TDR | 25,085,056 | 25,085,075 | CAGCCGTCCAGATGCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTGTGACAATACTGGGCTTG |
| SD | CAGGCATCTAGGATCTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 369 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 253 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10159A-B3 | VGB6 | C57BL/6NTac | |
| 10159A-F4 | VGB6 | C57BL/6NTac | |
| 10159A-C2 | VGB6 | C57BL/6NTac | |
