| Gene | A830007P12Rik |
| Chromosome | 2 |
| Deletion Start | 25,195,886 |
| Deletion End | 25,194,578 |
| Deletion Size | 1,309 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,195,417 | 25,195,436 | CTGCAGCGTTCTGTGTTTGG |
| TUP | 25,195,362 | 25,195,387 | CCCTGCTGCGAGACTACCTGGCTACA |
| TUR | 25,195,333 | 25,195,351 | GCCAGGAGCAAGGGATGAC |
| TDF | 25,194,919 | 25,194,937 | GCCATAGCGCAGGCAGTAC |
| TDP | 25,194,892 | 25,194,917 | CAGACTGAGGAATCTCCAGCCCAGGA |
| TDR | 25,194,861 | 25,194,880 | GGCCAGGAGCTCTCGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GCACTTCCTCACAGCCTAG |
| SD | GATGGACATAGTGGGAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 301 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10160A-A9 | VGB6 | C57BL/6NTac | |
| 10160A-C3 | VGB6 | C57BL/6NTac | |
| 10160A-D9 | VGB6 | C57BL/6NTac | |
| 10160A-G5 | VGB6 | C57BL/6NTac | |
| 10160A-G8 | VGB6 | C57BL/6NTac | |
| 10160A-E11 | VGB6 | C57BL/6NTac | |
