| Gene | Nxph2 |
| Chromosome | 2 |
| Deletion Start | 23,399,697 |
| Deletion End | 23,400,412 |
| Deletion Size | 716 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,399,730 | 23,399,752 | TGTGGACTGGGAAGACAGAACTG |
| TUP | 23,399,757 | 23,399,787 | TGAATGGAACAGATTTCCAACCAATGTTTCA |
| TUR | 23,399,789 | 23,399,810 | AGACGCAGAGGACTGGTAATCC |
| TDF | 23,400,240 | 23,400,260 | TTTCGACCCATCCAAAATCTG |
| TDP | 23,400,264 | 23,400,291 | CCAGGAGCAGACTCAGAGTCATGTGTCC |
| TDR | 23,400,294 | 23,400,319 | TGACCTTAAATGGTTTGGAACATAAC |
PCR Primers
| Primer | Sequence |
| SU | TTGATGTGCCTCATTTTAAC |
| SD | AGAAGCAATATTTCCAGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 468 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10161A-B5 | VGB6 | C57BL/6NTac | |
| 10161A-H9 | VGB6 | C57BL/6NTac | |
| 10161A-B4 | VGB6 | C57BL/6NTac | |
| 10161A-C12 | VGB6 | C57BL/6NTac | |
| 10161B-C3 | VGB6 | C57BL/6NTac | |
| 10161B-D9 | VGB6 | C57BL/6NTac | |
