| Gene | C1ql3 |
| Chromosome | 2 |
| Deletion Start | 13,010,844 |
| Deletion End | 13,004,246 |
| Deletion Size | 6,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,010,776 | 13,010,795 | CGTCGGCTCACTACGAGATG |
| TUP | 13,010,750 | 13,010,769 | ACCTGCCGCATGGTCTGCGA |
| TUR | 13,010,679 | 13,010,697 | TGGGCAGGGACTGCATAAG |
| TDF | 13,004,568 | 13,004,596 | GAAGTTGTTATGGAAATTCCTTCTAAACC |
| TDP | 13,004,539 | 13,004,565 | TGGGACCAACAGCAACATCAGTGTGCT |
| TDR | 13,004,514 | 13,004,537 | GACTTGCAGCCATAGCATACAATG |
PCR Primers
| Primer | Sequence |
| SU | CTCGGGACTTCAGTTCAAGT |
| SD | AGGACACCAAAGTATCATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 383 bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 425 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10163A-D1 | VGB6 | C57BL/6NTac | |
