| Gene | Thnsl1 |
| Chromosome | 2 |
| Deletion Start | 21,211,419 |
| Deletion End | 21,213,680 |
| Deletion Size | 2,262 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,211,443 | 21,211,466 | ACCTTGACCAGATGTCACCATTTG |
| TUP | 21,211,469 | 21,211,500 | ACAGATAGCACAGGAGTGTCTTTCTAGCCTGC |
| TUR | 21,211,502 | 21,211,521 | TGCGGCTCTGCACTTTAACC |
| TDF | 21,213,404 | 21,213,423 | AAGCTGCCCAGTGCTCATTG |
| TDP | 21,213,426 | 21,213,453 | TCCACAGCTCACTACTCCAAGTTTGCCC |
| TDR | 21,213,456 | 21,213,475 | CCCAGTGCCTGCATGATAGC |
PCR Primers
| Primer | Sequence |
| SU | ATGGAAATGGCACGATGAGG |
| SD | AAATATTTGTAAATGCTATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10164A-H6 | VGB6 | C57BL/6NTac | |
| 10164B-B12 | VGB6 | C57BL/6NTac | |
| 10164C-B12 | VGB6 | C57BL/6NTac | |
| 10164C-C1 | VGB6 | C57BL/6NTac | |
| 10164D-D1 | VGB6 | C57BL/6NTac | |
| 10164D-C4 | VGB6 | C57BL/6NTac | |
| 10164C-F4 | VGB6 | C57BL/6NTac | |
