| Gene | Slc34a3 |
| Chromosome | 2 |
| Deletion Start | 25,233,384 |
| Deletion End | 25,229,031 |
| Deletion Size | 4,354 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,233,345 | 25,233,364 | CCAGGTCCCCAACCCTACTC |
| TUP | 25,233,317 | 25,233,341 | CTCCGGTCCACCAGGTCAAAGGCAT |
| TUR | 25,233,291 | 25,233,313 | GACACCGAATACCTGCATTTCTC |
| TDF | 25,229,142 | 25,229,161 | CGCCTGGTGACTGCTTGTTG |
| TDP | 25,229,110 | 25,229,135 | CAGGGCCTGCAGCAACTCTCCTATGA |
| TDR | 25,229,085 | 25,229,106 | TCATAGCAGTGAGCCACTTTGC |
PCR Primers
| Primer | Sequence |
| SU | TCTGATAACCATCCGAGGTC |
| SD | CTACCCTAATCTCTAGTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 364 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10165C-A4 | VGB6 | C57BL/6NTac | |
| 10165C-B1 | VGB6 | C57BL/6NTac | |
| 10165C-B10 | VGB6 | C57BL/6NTac | |
| 10165C-C4 | VGB6 | C57BL/6NTac | |
| 10165C-C12 | VGB6 | C57BL/6NTac | |
| 10165C-D1 | VGB6 | C57BL/6NTac | |
