| Gene | Gm757 |
| Chromosome | 2 |
| Deletion Start | 25,236,336 |
| Deletion End | 25,235,850 |
| Deletion Size | 487 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,236,235 | 25,236,260 | ACTCTGCCTATGACCTCTCAGTACAC |
| TUP | 25,236,209 | 25,236,233 | TGCCTCGACGTCTCTACTGTGGCCA |
| TUR | 25,236,158 | 25,236,177 | TGTTCGTGGGCTGGCATATC |
| TDF | 25,236,140 | 25,236,159 | CAACACTGGATCCCCTGTCC |
| TDP | 25,236,113 | 25,236,137 | AGTGCCGACAAAGTACCCCTGTGCC |
| TDR | 25,236,093 | 25,236,111 | ACATGGTCACCCCTCCACG |
PCR Primers
| Primer | Sequence |
| SU | TAGGGGTGGGGTTAAGCAGG |
| SD | GCATAGACAGCAGGATGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10166B-A3 | VGB6 | C57BL/6NTac | |
| 10166B-E9 | VGB6 | C57BL/6NTac | |
| 10166B-F1 | VGB6 | C57BL/6NTac | |
| 10166B-H7 | VGB6 | C57BL/6NTac | |
| 10166B-A6 | VGB6 | C57BL/6NTac | |
