| Gene | Lrrc26 |
| Chromosome | 2 |
| Deletion Start | 25,289,972 |
| Deletion End | 25,291,060 |
| Deletion Size | 1,089 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,290,558 | 25,290,576 | GCCTGCCCTCGATGTGTTG |
| TUP | 25,290,585 | 25,290,607 | TGGCAACCCCTGGACGTGCAACT |
| TUR | 25,290,627 | 25,290,646 | GGTGCTTACGCAGCCAAGTG |
| TDF | 25,290,802 | 25,290,820 | GGATGCCGCCTTCAAACAG |
| TDP | 25,290,827 | 25,290,851 | CAGTCACTAGCAGCGCGAGACCTGG |
| TDR | 25,290,879 | 25,290,899 | TGGCCAAACTAGCAAGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGAACTGGTGGCTTTGATCG |
| SD | AGCTCTCCCAGTGTCCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10167A-D7 | VGB6 | C57BL/6NTac | |
| 10167A-F4 | VGB6 | C57BL/6NTac | |
| 10167A-A11 | VGB6 | C57BL/6NTac | |
| 10167A-G11 | VGB6 | C57BL/6NTac | |
| 10167A-D5 | VGB6 | C57BL/6NTac | |
