| Gene | Uap1l1 |
| Chromosome | 2 |
| Deletion Start | 25,365,612 |
| Deletion End | 25,361,625 |
| Deletion Size | 3,988 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,365,255 | 25,365,276 | CCGGAAGAGTTAGAGCCTATGG |
| TUP | 25,365,207 | 25,365,230 | CACTTCCCGAGTCCGTGTCCTCCA |
| TUR | 25,365,157 | 25,365,181 | GGTTACTAGGCACAGGAGGAAGAAG |
| TDF | 25,362,148 | 25,362,168 | GAGGTAGGTGCTGACCATTGC |
| TDP | 25,362,109 | 25,362,131 | CCCAAATGGAGACCCTCCAGCCA |
| TDR | 25,362,084 | 25,362,103 | GACACCAAGGGCGAGATCTC |
PCR Primers
| Primer | Sequence |
| SU | CAGAACAGTTTTATTCACGTG |
| SD | TAGACAGACAATCAGAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 455 bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 253 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10169A-E7 | VGB6 | C57BL/6NTac | |
| 10169B-H7 | VGB6 | C57BL/6NTac | |
| 10169B-B7 | VGB6 | C57BL/6NTac | |
