| Gene | Lcn9 |
| Chromosome | 2 |
| Deletion Start | 25,823,144 |
| Deletion End | 25,824,916 |
| Deletion Size | 1,773 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,823,939 | 25,823,960 | CCTGGTGCTTGCTCTGATCTTC |
| TUP | 25,823,962 | 25,823,986 | CCCCTCACTGTCCTCCAGGTTGCAA |
| TUR | 25,823,988 | 25,824,011 | CCATGGTCACAGCTACACATTCTC |
| TDF | 25,824,511 | 25,824,530 | ATGGGACCAAGACCCAAGTG |
| TDP | 25,824,559 | 25,824,583 | CCCAGTGGCTGGGATTCCCAAACAG |
| TDR | 25,824,588 | 25,824,607 | TCTAGGGCCCATGGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | ATAGCCAAACCCTGTTGGTC |
| SD | CAACCCAGTGATGAGCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 270 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10171A-B7 | VGB6 | C57BL/6NTac | |
| 10171A-B9 | VGB6 | C57BL/6NTac | |
| 10171A-C6 | VGB6 | C57BL/6NTac | |
| 10171A-F8 | VGB6 | C57BL/6NTac | |
