| Gene | 1700029I15Rik |
| Chromosome | 2 |
| Deletion Start | 92,382,932 |
| Deletion End | 92,383,586 |
| Deletion Size | 655 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,383,084 | 92,383,105 | TGGAAGAGGCTGACCATTATCC |
| TUP | 92,383,120 | 92,383,147 | TCATGAAGTCTTCACCCTGGACTGTCCC |
| TUR | 92,383,149 | 92,383,169 | GGCCATTGCCACTTCTTTGTG |
| TDF | 92,383,156 | 92,383,175 | AAGTGGCAATGGCCTTTTGG |
| TDP | 92,383,177 | 92,383,204 | TTAGGGCTCAGCACAGCAATATTTCCCA |
| TDR | 92,383,219 | 92,383,240 | GCTGAAAATTCCTCCGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | GTAGTCTAGAGTGATGGACAG |
| SD | AGACCCTGTTCTCATCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 372 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10173A-G3 | VGB6 | C57BL/6NTac | |
| 10173A-G5 | VGB6 | C57BL/6NTac | |
| 10173B-A2 | VGB6 | C57BL/6NTac | |
| 10173D-B6 | VGB6 | C57BL/6NTac | |
| 10173D-B8 | VGB6 | C57BL/6NTac | |
| 10173D-B11 | VGB6 | C57BL/6NTac | |
| 10173D-C8 | VGB6 | C57BL/6NTac | |
| 10173D-E2 | VGB6 | C57BL/6NTac | |
| 10173D-F6 | VGB6 | C57BL/6NTac | |
