| Gene | Trip13 |
| Chromosome | 13 |
| Deletion Start | 73,937,578 |
| Deletion End | 73,913,222 |
| Deletion Size | 24,357 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,937,195 | 73,937,217 | CTCACATTGCCGCATATCCCATC |
| TUP | 73,937,168 | 73,937,194 | CAGACCCTACCACCATCCCAGACTATC |
| TUR | 73,937,127 | 73,937,147 | GCTCCATAGGCCAGTCAGTTG |
| TDF | 73,913,901 | 73,913,919 | CCCCATGAGCCTGGTTCTG |
| TDP | 73,913,871 | 73,913,898 | CACACTCGATGGTGACCTGGAATCACTC |
| TDR | 73,913,849 | 73,913,869 | GCCTGGCTCTGAGCAGCTAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTCGCCACCGCCAAGTGC |
| SD | TTGTGTCTGACAGGCTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10174A-F5 | VGB6 | C57BL/6NTac | |
| 10174A-G5 | VGB6 | C57BL/6NTac | |
| 10174B-D7 | VGB6 | C57BL/6NTac | |
| 10174B-F8 | VGB6 | C57BL/6NTac | |
| 10174B-A10 | VGB6 | C57BL/6NTac | |
