| Gene | Ggnbp1 |
| Chromosome | 17 |
| Deletion Start | 27,029,444 |
| Deletion End | 27,036,216 |
| Deletion Size | 6,773 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,029,479 | 27,029,498 | CCCTCGGTCACGGATCTTAG |
| TUP | 27,029,500 | 27,029,523 | CTGCTCCTCCATGCTGCGCTTTCT |
| TUR | 27,029,525 | 27,029,544 | TTACTCCCCACCAGGTTTCG |
| TDF | 27,035,665 | 27,035,684 | GGCCTCTCCGGTAGCAAGTG |
| TDP | 27,035,687 | 27,035,710 | TCAGAGTCCCACGTCTCCCCACCA |
| TDR | 27,035,727 | 27,035,747 | CCTTAGCCCGTGGTCCTAAGG |
PCR Primers
| Primer | Sequence |
| SU | CCGCTTTCCTTCCAGTAGAC |
| SD | AGCCCAGGAGGTGAATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 290 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 255 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10175D-E3 | VGB6 | C57BL/6NTac | |
| 10175E-B1 | VGB6 | C57BL/6NTac | |
| 10175D-C3 | VGB6 | C57BL/6NTac | |
