| Gene | Prg3 |
| Chromosome | 2 |
| Deletion Start | 84,988,715 |
| Deletion End | 84,993,766 |
| Deletion Size | 5,052 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,989,383 | 84,989,404 | GAGGAGGCAATGGAGTCAGATC |
| TUP | 84,989,425 | 84,989,450 | TCTGCTTGCCCCAAGGAAGAGGACAC |
| TUR | 84,989,453 | 84,989,474 | CCAGGAGTTCCCTGAAAATGAG |
| TDF | 84,993,568 | 84,993,590 | GCAGGTTATTGCCACAATGTTTG |
| TDP | 84,993,597 | 84,993,625 | CCTTCCTTGGCTATCATCAGCTTCAGAGG |
| TDR | 84,993,635 | 84,993,656 | CACCCAACCCTTGTCAGATACC |
PCR Primers
| Primer | Sequence |
| SU | GAGGCAGCAGTAGCCCTCCC |
| SD | CTTTGTCACTGCTCCCCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 248 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10177A-E7 | VGB6 | C57BL/6NTac | |
| 10177A-B11 | VGB6 | C57BL/6NTac | |
| 10177A-G10 | VGB6 | C57BL/6NTac | |
