| Gene | Lcn10 |
| Chromosome | 2 |
| Deletion Start | 25,682,771 |
| Deletion End | 25,685,593 |
| Deletion Size | 2,823 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,683,068 | 25,683,089 | TTCACAGACACCTCCACAAAGG |
| TUP | 25,683,099 | 25,683,126 | ACTGCTTGGCTCTGCTGCTCCTCTTACA |
| TUR | 25,683,128 | 25,683,149 | CAGTCCTGCTTCAGGACACATC |
| TDF | 25,685,120 | 25,685,138 | GCCACCATCCTTCCGAAAG |
| TDP | 25,685,140 | 25,685,164 | CGGTACCACATGGCCTCAGGCTTTC |
| TDR | 25,685,188 | 25,685,208 | GGATGGACCAGCTGTCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | GAGAACAATGAGGTGTCCATC |
| SD | CTGAAGTCAGTGTTGACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10181B-H8 | VGB6 | C57BL/6NTac | |
| 10181B-G7 | VGB6 | C57BL/6NTac | |
