| Gene | Lcn13 |
| Chromosome | 2 |
| Deletion Start | 25,737,038 |
| Deletion End | 25,702,751 |
| Deletion Size | 34,288 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,700,160 | 25,700,180 | CCAGATGACCTGGTGGATGTG |
| TUP | 25,700,184 | 25,700,206 | CCACCACCCATTGTCCCTCCCAT |
| TUR | 25,700,210 | 25,700,229 | GCAGTAGGCCAGGCCATCTC |
| TDF | 25,702,565 | 25,702,587 | GTGTCCCTGGCTTCTGTATTGTC |
| TDP | 25,702,610 | 25,702,634 | TCTCACTGGGACCTCAGGCCTCTGC |
| TDR | 25,702,637 | 25,702,656 | CCTCCCACAGCCAAGATCTG |
PCR Primers
| Primer | Sequence |
| SU | CTGCAGGTGACACTGAGTTG |
| SD | CATAAGTAGCCAGGTCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 37300 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10182A-B2 | VGB6 | C57BL/6NTac | |
| 10182A-D5 | VGB6 | C57BL/6NTac | |
| 10182A-G12 | VGB6 | C57BL/6NTac | |
| 10182A-F1 | VGB6 | C57BL/6NTac | |
| 10182A-H5 | VGB6 | C57BL/6NTac | |
| 10182A-A8 | VGB6 | C57BL/6NTac | |
