| Gene | Lcn3 |
| Chromosome | 2 |
| Deletion Start | 25,765,557 |
| Deletion End | 25,767,991 |
| Deletion Size | 2,435 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,765,883 | 25,765,905 | GCCCAGGTACACTTGGAGTAAGC |
| TUP | 25,765,909 | 25,765,938 | CCAAAACATAGAAAGCTCACATGATCGGGA |
| TUR | 25,765,943 | 25,765,965 | TTCATGTCTGCCCATTCTATTCC |
| TDF | 25,767,838 | 25,767,860 | CTTGGTATTCAACAGGGCTTCAG |
| TDP | 25,767,865 | 25,767,888 | CACCTGGAAGCCTCAGTTGCAGCC |
| TDR | 25,767,891 | 25,767,912 | GGACACAGAACCTGGGAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TCTTGAAGTCTTAACAAGCTG |
| SD | AGAAAGGCTGGGAGCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 306 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10184A-B3 | VGB6 | C57BL/6NTac | |
| 10184A-G8 | VGB6 | C57BL/6NTac | |
| 10184B-D3 | VGB6 | C57BL/6NTac | |
| 10184C-A3 | VGB6 | C57BL/6NTac | |
